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Transcription from maize storage protein gene promoters in yeast.
Author(s) -
Langridge P.,
Eibel H.,
Brown J.W.,
Feix G.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb02157.x
Subject(s) - biology , gene , yeast , promoter , saccharomyces cerevisiae , genetics , transcription (linguistics) , endosperm , gene expression , linguistics , philosophy
Genes coding for zein storage proteins of maize of the 19 000‐ and 21 000‐dalton size classes were integrated into chromosome II of the yeast Saccharomyces cerevisiae. Using the transformed yeast strains as an in vivo transcription system it was shown that promoter regions of the zein genes of both size classes were accurately recognized. In particular, the zein mRNA synthesis in yeast starts at the same nucleotide positions used in the maize endosperm. A difference in the promoter activity between the 21 000‐ and the 19 000‐dalton zein genes was observed.