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Alternative splicing in the 5′ moiety of the H‐2Kd gene transcript.
Author(s) -
Transy C.,
Lalanne J.L.,
Kourilsky P.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb02143.x
Subject(s) - biology , gene , microbiology and biotechnology , complementary dna , rna splicing , rna , alternative splicing , gene expression , genetics , messenger rna
The H‐2Kd gene, which encodes a mouse major transplantation antigen, was transfected into L TK‐ mouse fibroblasts. Two transcripts of the gene were detected by S1 nuclease mapping analysis. They correspond to two previously characterized cDNA clones isolated from DBA/2 mouse liver RNA, leading to the conclusion that the H‐2Kd gene gives rise to two distinct transcripts through an alternate use of splicing sites. The non‐canonical RNA potentially encodes a so far undescribed H‐2Kd‐like molecule. It is present in all tissues tested (liver, spleen, thymus, kidney) albeit in lower amounts (approximately 10‐fold less) than the canonical RNA coding for H‐2Kd.