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Is actin a transcription initiation factor for RNA polymerase B?
Author(s) -
Egly J.M.,
Miyamoto N.G.,
Moncollin V.,
Chambon P.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb02141.x
Subject(s) - biology , rna polymerase ii , sigma factor , transcription factor ii d , polymerase , transcription factor ii f , rna polymerase , microbiology and biotechnology , rna polymerase i , transcription (linguistics) , genetics , rna dependent rna polymerase , rna , promoter , gene expression , gene , linguistics , philosophy
We have previously reported that two fractions derived from HeLa cell S100 extracts, the heparin flow‐through and the heparin 0.6 M KCl eluate are required in vitro for efficient and accurate transcription by RNA polymerase class B (II). We have further purified a factor present in the heparin flow‐through fraction, which markedly stimulates specific transcription catalyzed by the heparin 0.6 M KCl eluate. We report here that some of the properties of the stimulatory factor present in our most purified fractions are strikingly similar to those of actin. We demonstrate also that this factor acts at the pre‐initiation level of the transcription reaction.