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Cosmid clones derived from both euchromatic and heterochromatic regions of the human Y chromosome.
Author(s) -
Wolfe J.,
Erickson R.P.,
Rigby P.W.,
Goodfellow P.N.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb02081.x
Subject(s) - cosmid , biology , euchromatin , homology (biology) , genetics , heterochromatin , chromosome , chromosome 16 , y chromosome , microbiology and biotechnology , x chromosome , dna , gene
Clones containing sequences derived from the human Y chromosome have been isolated from cosmid libraries of a human‐mouse hybrid cell line. These libraries were constructed in the new expression vectors Homer V and Homer VI. The collection of cosmids isolated is enriched for unique sequence DNA and only a few of the cosmids contain the tandemly repeated sequences which constitute a major portion of the Y chromosome. Three cosmids have been studied in detail. One cosmid shows extensive homology over at least 20 kb with the long arm of the X chromosome; this homology is outside the predicted homology region required for sex chromosome pairing. The other two clones contain unique sequences specific to the Y chromosome and both map to the heterochromatic region of the Y chromosome long arm.