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Structure and in vitro transcription of a glycine tRNA gene from Bombyx mori.
Author(s) -
Fournier A.,
Guérin M.A.,
Corlet J.,
Clarkson S.G.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb02009.x
Subject(s) - biology , bombyx mori , transfer rna , transcription (linguistics) , gene , genetics , in vitro , microbiology and biotechnology , rna , linguistics , philosophy
We report the sequences of a Bombyx mori tRNA1Gly gene, its flanking regions, and its in vitro transcription products. The 5′ flanking DNA contains the sequences TATAC, TATTTT and TTC located 30, 18 and 4 nucleotides, respectively, in front of the transcription initiation site. These resemble, in both position and composition, sequences preceding other RNA polymerase III genes of B. mori. A deletion mutant retaining these conserved sequences and an additional 8 bp of flanking DNA is transcribed better than the wild‐type gene in cell‐free extracts from Xenopus laevis and B. mori. A mutant lacking the conserved sequences is expressed in the frog extract, but is inactive in the homologous system.