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Cell surface expression of fusogenic vesicular stomatitis virus G protein from cloned cDNA.
Author(s) -
Riedel H.,
KondorKoch C.,
Garoff H.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb01999.x
Subject(s) - vesicular stomatitis virus , biology , complementary dna , microbiology and biotechnology , fusion protein , virus , virology , glycoprotein , vesicular stomatitis , cell , rous sarcoma virus , biochemistry , recombinant dna , gene
Vesicular stomatitis virus (VSV) enters the host cell by the receptor‐mediated endocytotic pathway. This brings the virus particle into acidic vesicles inside the cell where infection occurs through a fusion event between the viral and the host vesicle membrane. In this work we have shown that the VSV glycoprotein (G) carries the fusion activity of this virus. The G protein was expressed on the surface of baby hamster kidney 21 cells from cloned cDNA which had been engineered into an expression vector and introduced into cell nuclei with the aid of a glass microneedle. A short (60 s) treatment with acid (pH less than or equal to 6.0) medium induced fusion of cells having G protein on their surface. For efficient G protein expression and cell‐cell fusion we had to trim the 5′ end of the G cDNA and to use as promoter the long terminal repeat of the mouse Moloney sarcoma virus.