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Role of cell division in differentiation of myoblasts infected with a temperature‐sensitive mutant of Rous sarcoma virus.
Author(s) -
Falcone G.,
Boettiger D.,
Alemà S.,
Tatò F.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb01971.x
Subject(s) - rous sarcoma virus , biology , myogenesis , cell division , myocyte , mutant , microbiology and biotechnology , virology , virus , cell , genetics , gene
The relationship between a potential requirement for cell DNA synthesis and the expression of differentiated muscle cell functions was investigated using primary chicken embryo myoblasts infected with a temperature‐sensitive mutant of Rous sarcoma virus (RSV). Under optimized conditions, transformed myoblasts growing at the permissive temperature could differentiate into multinucleated myotubes, express muscle‐specific myosin, desmin and acetylcholine receptors in the absence of DNA synthesis and cell division following a shift to the non‐permissive temperature. Furthermore, the experiments demonstrate that individual RSV‐infected myoblasts have two options: either to divide and express the transformed phenotype or to withdraw from the cell cycle and differentiate into myotubes. The choice between these options appears to depend on the protein‐kinase activity of pp60src, the src gene product.