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Assembly of transfected DNA into chromatin: structural changes in the origin‐promoter‐enhancer region upon replication.
Author(s) -
Cereghini S.,
Yaniv M.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb01959.x
Subject(s) - chromatin , biology , origin recognition complex , dna replication , dna , microbiology and biotechnology , scaffold/matrix attachment region , enhancer , micrococcal nuclease , plasmid , origin of replication , transfection , eukaryotic dna replication , genetics , gene , nucleosome , chromatin remodeling , gene expression
Chimeric SV40 DNA containing only the early region, or plasmid DNA harboring the origin‐promoter‐enhancer region of SV40, when introduced into CV‐1 or Cos‐1 monkey cells by DEAE‐dextran mediated transfer are rapidly assembled in a typical chromatin structure revealed by the generation of a regular 190 bp repeat ladder after micrococcal nuclease digestion. DNA replication is not required for this assembly process. Chromatin‐specific DNase I hypersensitive sites are observed in the enhancer region of these minichromosomes. The pattern of the sites differs between non‐replicating and post‐replicated chromatin. The latter is identical to that observed in the lytic cycle. The presence of large T antigen is not sufficient for the shift in the structure of the chromatin. These experiments suggest that replication can modulate protein‐DNA interactions during viral infection or upon cell differentiation.