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The chromosomal integration site determines the tissue‐specific methylation of mouse mammary tumour virus proviral genes.
Author(s) -
Günzburg W.H.,
Groner B.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb01941.x
Subject(s) - biology , gene , methylation , dna methylation , mouse mammary tumor virus , provirus , virus , virology , genetics , cancer research , microbiology and biotechnology , gene expression , genome
Multiple endogenous mouse mammary tumour virus (MMTV) proviral genes are present at different chromosomal locations in inbred mouse strains. Proviral DNA methylation is location and tissue specific. The methylation patterns are stably inherited and appear to be conferred upon the viral DNA by the flanking mouse genomic DNA. In transformed cells, either mammary carcinoma cells, or cells immortalized by SV40 in vitro, the stable pattern of methylation is lost. Although hypomethylation of proviral genes, both in normal and in transformed tissue, accompanies MMTV‐specific RNA expression, it is also observed in non‐expressing tissues.