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Yeast RNA polymerase II initiates transcription in vitro at TATA sequences proximal to potential non‐B forms of the DNA template.
Author(s) -
Lescure B.,
Arcangioli B.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb01929.x
Subject(s) - biology , transcription factor ii d , polymerase , transcription (linguistics) , dna , yeast , microbiology and biotechnology , tata box , genetics , rna polymerase ii , rna polymerase iii , rna polymerase , rna , gene , rna dependent rna polymerase , promoter , gene expression , linguistics , philosophy
Pure yeast RNA polymerase II selectively initiates an abortive in vitro transcript within a TATA box of the yeast iso‐1 cytochrome c gene promoter. Using a series of promoter deletions we show that a DNA sequence located upstream of the TATA box is needed for an efficient in vitro transcription. Supercoiling of the DNA template is an absolute requirement for the specific in vitro transcription. Examination of the DNA structure near several in vitro initiation sites shows that the common features observed are the presence of a TATA sequence in which RNA synthesis is initiated, and which is proximal to a potential non‐B form of the DNA (a B to Z transition or a cruciform structure).