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The DNA sequence of the nuclear gene coding for the 17‐kd subunit VI of the yeast ubiquinol‐cytochrome c reductase: a protein with an extremely high content of acidic amino acids.
Author(s) -
Van Loon A.P.,
De Groot R.J.,
De Haan M.,
Dekker A.,
Grivell L.A.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb01924.x
Subject(s) - biology , ubiquinol , gc content , gene , open reading frame , reductase , protein subunit , cytochrome c , genetics , microbiology and biotechnology , biochemistry , peptide sequence , enzyme , coenzyme q – cytochrome c reductase , mitochondrion , genome
We have determined the DNA sequence of the nuclear gene coding for the 17‐kd subunit VI of the ubiquinol‐cytochrome c reductase. The reading frame found encodes a putative polypeptide of 17 394 daltons. This protein is highly unusual: 38% of its residues are acidic and 14% are basic amino acids. The most notable feature in the protein sequence is a stretch of 25 consecutive acidic amino acids. The polypeptide has homology with the 9‐kd so‐called ‘hinge’ protein of beef‐heart complex III, which also has a cluster of acidic residues. Acidic amino acids are likely to be essential for the function of these proteins, since their degree of conservation is higher than that of other residues.