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Autogenous production of interferon‐beta switches on HLA genes during differentiation of histiocytic lymphoma U937 cells.
Author(s) -
Yarden A.,
ShureGottlieb H.,
Chebath J.,
Revel M.,
Kimchi A.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb01915.x
Subject(s) - biology , gene , interferon beta , human leukocyte antigen , beta (programming language) , u937 cell , interferon , histiocyte , genetics , immunology , cell culture , antigen , computer science , programming language
The expression of class I HLA genes was measured during the in vitro differentiation of human U937 lymphoma cells towards macrophages. Following the onset of differentiation by phorbol myristate acetate the levels of cytoplasmic mRNA that hybridized with a [32P]HLA‐B cDNA probe increased by a factor of nine. Elevation in HLA mRNA accumulation was followed by an increase in the rate of synthesis of HLA proteins and also by a dramatic increase in class I HLA cell surface antigen expression, as shown by cytofluorimetric analysis. The elevation in HLA mRNA and surface antigens could be prevented by adding antibodies against human interferon‐beta (IFN‐beta) to the culture medium at the onset of differentiation. Interferon antiviral activity was detected in the medium of differentiated U937 cells. The same anti‐IFN‐beta antibodies prevented the increase in (2′‐5′)oligo(A) synthetase activity which also takes place in differentiating U937 cells. Accumulation of the IFN‐induced (2′‐5′)oligo(A) synthetase in U937 cells is preceded by an increase in its specific 1.6‐kb mRNA as shown by hybridization to cloned (2′‐5′)‐oligo(A) synthetase cDNA. The enzyme was preferentially found in the nuclear fraction of differentiating U937 cells. We suggest that an autogenous production of interferon‐beta by the differentiating cells, switches on expression of the class I HLA genes as well as that of the (2′‐5′)oligo(A) synthetase.