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Differential binding of a S. cerevisiae RNA polymerase III transcription factor to two promoter segments of a tRNA gene.
Author(s) -
Stillman D.J.,
Geiduschek E.P.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb01895.x
Subject(s) - biology , transcription (linguistics) , rna polymerase , promoter , rna polymerase ii , gene , microbiology and biotechnology , genetics , rna polymerase iii , polymerase , rna , gene expression , philosophy , linguistics
A Saccharomyces cerevisiae protein fraction which binds specifically to the internal promoter regions of genes that are transcribed by RNA polymerase III is shown to function as a transcription factor. We postulate that the stable DNA binding of the factor confers stability on polymerase III transcription complexes. Analysis of the binding by DNase ‘foot‐printing’ distinguishes three segments of the S. cerevisiae tRNALeu3 gene: a region surrounding the so‐called A block of the internal promoter, a region surrounding the B block and an intermediate segment. Binding to the A and B block regions is connected, but the B block region exerts a dominant effect.