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Inhibition of N‐linked oligosaccharide trimming does not interfere with surface expression of certain integral membrane proteins.
Author(s) -
Burke B.,
Matlin K.,
Bause E.,
Legler G.,
Peyrieras N.,
Ploegh H.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb01845.x
Subject(s) - biology , vesicular stomatitis virus , glycoprotein , oligosaccharide , proteases , virus , hemagglutinin (influenza) , membrane glycoproteins , membrane protein , vesicular stomatitis indiana virus , antigen , biochemistry , virology , membrane , enzyme , genetics
The effects of 1‐deoxynojirimycin (dNM) and 1‐deoxymannojirimycin (dMM), inhibitors of oligosaccharide trimming glucosidase I and mannosidase I, respectively, on the biosynthesis of vesicular stomatitis virus G protein, influenza virus hemagglutinin, and human class I histocompatibility antigens were investigated. Although the oligosaccharides of these membrane glycoproteins were greatly altered, neither dNM nor dMM interferred with their surface expression, as determined by a variety of assays, including accessibility to proteases and antibodies; neither did these drugs inhibit production of infectious virus particles.