Premium
The Schizosaccharomyces pombe sup3‐i suppressor recognizes ochre, but not amber codons in vitro and in vivo.
Author(s) -
Hottinger H.,
Stadelmann B.,
Pearson D.,
Frendewey D.,
Kohli J.,
Söll D.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb01823.x
Subject(s) - schizosaccharomyces pombe , biology , suppressor , genetics , gene , saccharomyces cerevisiae
The inefficient suppressor sup3‐i of the fission yeast Schizosaccharomyces pombe is an ochre suppressor. Sup3‐i was derived from the efficient serine inserting UGA suppressor sup3‐e. The cloning and sequencing of the sup3‐i gene indicate that the suppressor is different from the parent sup3‐e by a C–‐T substitution in the sequence coding for the middle position of the anticodon. In vitro translation assays supplemented with purified sup3‐i tRNA and programmed with Xenopus globin mRNAs lead to the accumulation of a readthrough product in response to UAA termination signals, but not in response to UGA termination codons. Transformation of Saccharomyces cerevisiae nonsense mutant strains with plasmid DNA carrying the S. pombe sup3‐i gene, led to ochre, but not amber or UGA suppression in vivo.