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Two‐dimensional crystalline sheets of Bacillus stearothermophilus 50S ribosomal particles.
Author(s) -
Arad T.,
Leonard K.,
Wittmann H.G.,
Yonath A.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb01772.x
Subject(s) - biology , bacillus (shape) , ribosomal rna , geobacillus stearothermophilus , 50s , ribosome , thermophile , microbiology and biotechnology , biochemistry , genetics , bacteria , rna , gene
Two‐dimensional crystalline sheets of the large ribosomal subunit from Bacillus stearothermophilus have been obtained using a slightly modified procedure to that for growing three‐dimensional crystals of the same material. The crystalline subunits are packed within monolayers in a relatively small unit cell, the dimensions of which are closely related to those observed for two forms of the three‐dimensional crystals. The packing symmetry is p121, and the optical diffraction patterns of micrographs of negatively stained crystals extend to approximately 3.0 nm.