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Cloning and sequencing of a full length cDNA coding for a human apoferritin H chain: evidence for a multigene family.
Author(s) -
Costanzo F.,
Santoro C.,
Colantuoni V.,
Bensi G.,
Raugei G.,
Romano V.,
Cortese R.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb01756.x
Subject(s) - complementary dna , computer science , cloning (programming) , coding region , coding (social sciences) , biology , gene , genetics , programming language , sociology , social science
We have cloned a segment of cDNA from human liver coding for an apoferritin subunit, probably an H chain. Sequence comparison with the available protein sequence shows that our clone corresponds to a ferritin subunit present as a minor species in human spleen and placenta, but as major species in HeLa cells. Northern blot analysis shows the existence of only one band of similar size in human liver, HeLa cells, Daudi lymphoma and Hep3B hepatoma cell lines. In contrast, Southern blot analysis provides evidence for a multigene family.