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The normal developmental regulation of a cloned sgs3 ‘glue’ gene chromosomally integrated in Drosophila melanogaster by P element transformation.
Author(s) -
Richards G.,
Cassab A.,
Bourouis M.,
Jarry B.,
Dissous C.
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01714.x
Subject(s) - biology , drosophila melanogaster , p element , transformation (genetics) , genetics , drosophilidae , gene , dna transposable elements , transposable element , microbiology and biotechnology , genome
A 7‐kb genomic segment containing the coding sequence for the Drosophila melanogaster Formosa variant of salivary gland secretion protein 3 (sgs3) has been inserted into the snw y, bw, st strain of D. melanogaster using the P transformation vector p.6.1. The inserted sequence codes for a shorter RNA which can be distinguished from that of the host gene. The amount of RNA, and its stage‐ and tissue‐specific synthesis is identical to that of the host gene, which suggests that all the cis‐acting regulatory DNA sequences for this gene are contained within this 7‐kb segment.