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‘Attached cell’ antigen 28.3.7 mapping to human chromosome 15 characterises TPA‐induced differentiation of the promyelocytic HL‐60 cell line to give macrophage/monocyte populations.
Author(s) -
Blaineau C.,
Avner P.,
Tunnacliffe A.,
Goodfellow P.
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01692.x
Subject(s) - biology , antigen , microbiology and biotechnology , cell culture , monocyte , genetics
Human cells growing in vitro attached to the substratum express a cell antigen called 28.3.7 identified by a species‐specific monoclonal antibody. This antigen is not expressed on human cells growing in suspension. The antigen has a mol. wt. in reduced SDS‐polyacrylamide gel electrophoresis gels of 95 000 and in human‐mouse somatic cell hybrids, expression of the antigen is controlled by a gene, MIC7, mapping to human chromosome 15. The antigen functions as a marker for macrophage differentiation. In vitro differentiation of the 28.3.7 antigen‐negative human promyelocytic leukaemia line HL‐60 induced by phorbol ester, results in the formation of a macrophage/monocyte population and the concomitant expression of the 28.3.7 antigen on this adherent cell population.