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Coding and 3′ non‐coding nucleotide sequence of chalcone synthase mRNA and assignment of amino acid sequence of the enzyme
Author(s) -
Reimold Ursula,
Kröger Manfred,
Kreuzaler Fritz,
Hahlbrock Klaus
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01661.x
Subject(s) - biology , chalcone synthase , coding region , nucleic acid sequence , peptide sequence , genetics , sequence (biology) , biochemistry , enzyme , microbiology and biotechnology , gene , biosynthesis
The nucleotide sequence of an almost complete cDNA copy of chalcone synthase mRNA from cultured parsley cells ( Petroselinum hortense ) has been determined. The cDNA copy comprised the complete coding sequence for chalcone synthase, a short A‐rich stretch of the 5′ non‐coding region and the complete 3′ non‐coding region including a poly(A) tail. The amino acid sequence deduced from the nucleotide sequence of the cDNA is consistent with a partial N‐terminal sequence analysis, the total amino acid composition, the cyanogen bromide cleavage pattern, and the apparent mol. wt. of the subunit of the purified enzyme.