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Easy identification of cDNA clones.
Author(s) -
Rüther U.,
MüllerHill B.
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01659.x
Subject(s) - complementary dna , cdna library , biology , cloning (programming) , microbiology and biotechnology , genetics , genomic library , library , gene , computational biology , base sequence , computer science , programming language , 16s ribosomal rna
A set of six cloning vectors, pUR 278, 288, 289, 290, 291, 292 is presented. These vectors have the cloning sites, BamHI, SalI, PstI, XbaI and HindIII, in all frames at the 3′ end of the lacZ gene. Insertion of cDNA in the proper cloning sites leads to a fusion protein of active beta‐galactosidase and the peptide encoded by the cDNA. A simple immunoenzymatic assay can be used to identify clones in such a cDNA library.