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A transformation system for the hypotrichous ciliate Stylonychia mytilus
Author(s) -
Wünning Ingrid U.,
Lipps Hans J.
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01653.x
Subject(s) - reprint , biology , humanities , philosophy , physics , astronomy
We describe a transformation system for the ciliate Stylonychia mytilus . The neomycin resistance gene from Escherichia coli transposon Tn5, which codes for the enzyme phosphotransferase and confers resistance to the antibiotic G 418, was ligated into macronuclear ‘gene‐size’ DNA molecules. Using this recombinant DNA for transformation experiments we show that the gene is replicated and expressed in transformed cells.