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Correlation between endogenous nucleosomal hyper(ADP‐ribosyl)ation of histone H1 and the induction of chromatin relaxation.
Author(s) -
Aubin R.J.,
Fréchette A.,
Murcia G.,
Mandel P.,
Lord A.,
Grondin G.,
Poirier G.G.
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01643.x
Subject(s) - biology , histone h1 , chromatin , histone , endogeny , correlation , nucleosome , genetics , microbiology and biotechnology , dna , biochemistry , geometry , mathematics
The effect of poly(ADP‐ribose) synthesis on chromatin structure was investigated by velocity sedimentation and electron microscopy. We demonstrate that locally relaxed regions can be generated within polynucleosome chains by the activity of their intrinsic poly(ADP‐ribose)polymerase. This relaxation phenomenon is also shown to be NAD dependent and to be correlated with the formation of hyper(ADP‐ribosyl)ated forms of histone H1. Evidence is also presented which suggests that hyper(ADP‐ribosyl)ated histone H1 is neither released from the relaxed chromatin, nor does it seem to participate in polynucleosomal aggregation.