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A monoclonal antibody to the heavy chain of clathrin.
Author(s) -
Louvard D.,
Morris C.,
Warren G.,
Stanley K.,
Winkler F.,
Reggio H.
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01640.x
Subject(s) - immunoelectron microscopy , clathrin , monoclonal antibody , clone (java method) , polyclonal antibodies , biology , microbiology and biotechnology , vesicle , antibody , genetics , gene , membrane
Monoclonal antibodies have been raised to pig brain triskelions and one clone, DC41, was found to recognize the clathrin heavy chain by immunoblotting. However, both by immunofluorescence and immunoelectron microscopy, and in complete contrast to polyclonal anti‐clathrin antibodies, monoclonal DC41 did not label either coated pits or coated vesicles anywhere in the cell. Instead it appeared to label the cell cytoplasm. These data suggest that DC41 recognizes a cytoplasmic form of clathrin, perhaps that form produced by uncoating of coated vesicles which is then ready to re‐build another coated pit.