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Analysis of sequences conferring autonomous replication in baker's yeast.
Author(s) -
Kearsey S.
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01626.x
Subject(s) - biology , autonomously replicating sequence , gene , genetics , computational biology , replication (statistics) , saccharomyces cerevisiae , homology (biology) , yeast , origin of replication , sequence analysis , dna replication , virology
A method is presented for rapid sequencing and mapping of elements which support autonomous replication in yeast. The strategy relies on a novel phage M13 vector which allows detection of ARS (autonomously replicating sequence) function in cloned fragments. Deletion mapping of an ARS element linked to the HO gene of Saccharomyces cerevisiae has identified a 57‐bp region 3′ to the gene, which is essential for autonomous replication. This region shows sequence homology to other ARS elements.