Premium
Two specific markers for neural differentiation of embryonal carcinoma cells.
Author(s) -
Eddé B.,
Jakob H.,
Darmon M.
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01610.x
Subject(s) - embryonal carcinoma , biology , microbiology and biotechnology , genetics , gene , cellular differentiation
Two multipotential embryonal carcinoma (EC) cell lines, 1003 and 1009, can be induced to form preferentially neural derivatives in vitro. Synthesis of specific proteins during neural differentiation was followed by two‐dimensional gel electrophoresis. The comparison of protein patterns obtained with neural and non‐neural derivatives of these EC cell lines indicates that two changes are specific for the neural pathway: (i) the appearance of a new beta‐tubulin isoform and (ii) the accumulation of the brain isozyme of creatine phosphokinase already present in small amounts in EC stem cells. These changes were found to take place early in the course of differentiation and to occur even when neurite outgrowth was prevented.