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Developmental changes in translatable mRNAs for the cerebral enolase isozymes alphaalpha and gammagamma.
Author(s) -
Zeitoun Y.,
Lamandé N.,
Keller A.,
Gros F.,
LegaultDemare L.
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01606.x
Subject(s) - biology , humanities , microbiology and biotechnology , philosophy
Using the rabbit reticulocyte cell‐free translation system we have estimated during ontogenesis the proportions of in vitro translatable alpha and gamma brain enolase mRNAs, which are two minor mRNA species. No polypeptide precursor to these enzyme subunits appears to be synthesized during translation in vitro. During brain development, the changes in translatable alpha and gamma mRNA content seem to parallel those of the corresponding antigens. The proportion of each of the enolase mRNAs is highest in adult mouse brain. Mechanisms controlling alpha and gamma antigen expression are discussed. In order to prepare the specific cDNA probes, purification of alpha and gamma mRNAs was undertaken.