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The outer membrane of yeast mitochondria: isolation of outside‐out sealed vesicles
Author(s) -
Riezman Howard,
Hay Rick,
Gasser Susan,
Daum Günther,
Schneider Greti,
Witte Christine,
Schatz Gottfried
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01553.x
Subject(s) - biology , bacterial outer membrane , vesicle , mitochondrion , yeast , membrane , inner mitochondrial membrane , protease , microsome , translocase of the inner membrane , biochemistry , mitochondrial carrier , inner membrane , saccharomyces cerevisiae , translocase of the outer membrane , mitochondrial membrane transport protein , enzyme , escherichia coli , gene
The yeast mitochondrial outer membrane was isolated and 10 of its major polypeptides were identified (mol. wts. 109, 70, 57, 45, 45, 42, 33, 29, 25 and 14 kd). The membrane has no major polypeptide in common with either mitochondrial inner membrane or rough microsomes. Protease treatment and immunochemical techniques showed that virtually all of the isolated outer membrane vesicles are sealed and display the same surface orientation as in the intact mitochondrion.