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Structure and expression of the mouse beta 2‐microglobulin gene isolated from somatic and non‐expressing teratocarcinoma cells.
Author(s) -
Daniel F.,
Morello D.,
Le Bail O.,
Chambon P.,
Cayre Y.,
Kourilsky P.
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01546.x
Subject(s) - biology , teratocarcinoma , microbiology and biotechnology , syngenic , beta (programming language) , mutant , somatic cell , embryonal carcinoma , gene , cosmid , complementary dna , gene expression , cell culture , cdna library , cellular differentiation , genetics , in vitro , computer science , programming language
Mouse teratocarcinoma cells express neither H‐2 heavy chains nor beta 2‐microglobulin (beta 2‐m). We have constructed two genomic libraries, one from PCC4‐aza‐RI embryonal carcinoma cells and the other from their adult syngenic counterpart 129/Sv liver cells (H‐2bc). The libraries were screened with a full length mouse beta 2‐m cDNA probe which we isolated and sequenced. Two cosmid clones carrying the entire beta 2‐m gene were isolated, one from each library. There was no detectable difference in structure between the two genes. Furthermore, both were shown to be active and to restore beta 2‐m synthesis upon transfer into mutant cells deficient in beta 2‐m. Irreversible DNA alterations in or around the beta 2‐m gene are thus unlikely to account for the lack of beta 2‐m gene expression in embryonal teratocarcinoma cells.