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Microdissection and cloning of the white locus and the 3B1‐3C2 region of the Drosophila X chromosome
Author(s) -
Pirrotta V.,
Hadfield C.,
Pretorius G.H.J.
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01523.x
Subject(s) - biology , locus (genetics) , genetics , white (mutation) , cloning (programming) , chromosome , gene , computer science , programming language
Fragments from the 3B‐3C region of the Drosophila X chromosomes were microdissected from salivary gland squashes and their DNA was cloned by the method developed by Scalenghe et al. (1981). These clones were used as starting points for a chromosome walk which covers 200 kb including bands 3B2 to 3C2. A number of deletion breakpoints were mapped on the cloned DNA allowing the localisation of several genes in the 3B region. The white locus in particular was isolated by microcloning the w a insertion site. Two transcripts, of 2 and 2.4 kb, respectively, arise from the white region and its vicinity.