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Regulation of MHC class II invariant chain expression: induction of synthesis in human and murine plasmocytoma cells by arresting replication.
Author(s) -
Rahmsdorf H.J.,
Koch N.,
Mallick U.,
Herrlich P.
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01507.x
Subject(s) - biology , cell culture , mhc class ii , microbiology and biotechnology , major histocompatibility complex , genetics , gene
The expression of the H2 Ia‐associated invariant chain (Ii) has been determined by pulse labeling cells with [35S]methionine and resolving the proteins. Expression is maximal in noncycling peripheral B lymphocytes and is reduced upon maturation of B lymphocytes to plasma cells. B cell‐derived cell lines behave correspondingly: IgM+ non‐secretor cell lines synthesize Ii while plasmocytoma cells do not. Pre‐B cell lines are also negative. In all Ii‐negative cell lines, the synthesis of Ii is selectively induced by treating the cells with inhibitors of replication.