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Rat prostatic steroid binding protein: DNA sequence and transcript maps of the two C3 genes.
Author(s) -
Hurst H.C.,
Parker M.G.
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01498.x
Subject(s) - biology , gene , intron , homology (biology) , genetics , tata box , nucleic acid sequence , transcription (linguistics) , microbiology and biotechnology , dna , regulatory sequence , gene expression , promoter , linguistics , philosophy
In the rat there are two non‐allelic genes C3(1) and C3(2) for the C3 polypeptide of prostatic steroid binding protein. We have cloned and sequenced both genes and show that only C3(1) is responsible for the production of authentic C3. Although there is a marked difference in their transcriptional activity, the two genes share extensive DNA sequence homology there being only one base difference from nucleotide ‐ 235 to within the first intron. Transcript mapping has shown that there are two distinct C3 transcripts which share a unique 3′ terminus but have 5′ termini 38 bases apart each preceded by a ‘TATA’ box homology. Interestingly, an identical repetitive element is present just upstream of both genes. Both families of transcripts, which are produced in a ratio of 18:1, are coordinately regulated by testosterone.