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Ligation of endogenous tRNA 3′ half molecules to their corresponding 5′ halves via 2′‐phosphomonoester,3′,5′‐phosphodiester bonds in extracts of Chlamydomonas 1
Author(s) -
Tyc Kazimierz,
Kikuchi Yo,
Konarska Maria,
Filipowicz Witold,
Gross Hans J.
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01470.x
Subject(s) - biology , phosphodiester bond , transfer rna , chlamydomonas , biochemistry , stereochemistry , rna , gene , chemistry , mutant
tRNA preparations from Chlamydomonas and wheat germ contain small amounts of tRNA 5′ halves and corresponding 3′ halves. Incubation of cell‐free extracts from the two sources with [γ‐ 32 P]ATP yielded 5′‐ 32 P‐labeled tRNA 3′ halves which were joined to their corresponding 5′ counterparts to form mature tRNA containing 2′‐phosphomonoester,3′, 5′‐phosphodiester bonds. tRNA 3′ halves labelled with T4 kinase were purified, sequenced and also joined to their 5′ counterparts. It is proposed that these tRNA halves may be intermediates of the tRNA splicing process, and that the RNA kinase and ligase activities observed here are part of the tRNA splicing complex.