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Sequence of the lacZ gene of Escherichia coli.
Author(s) -
Kalnins A.,
Otto K.,
Rüther U.,
MüllerHill B.
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01468.x
Subject(s) - biology , escherichia coli , lac operon , sequence (biology) , escherichia coli proteins , genetics , gene , enterobacteriaceae , base sequence , bacterial protein , microbiology and biotechnology
The nucleotide sequence of the lacZ gene coding for beta‐galactosidase (EC 3.2.1.23) in Escherichia coli has been determined. Beta‐Galactosidase is predicted to consist of 1023 residues, resulting in a protein with a mol. wt. of 116 353 per subunit. The protein sequence originally determined by Fowler and Zabin was shown to be essentially correct and in an Appendix these authors comment on the discrepancies.