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Detection of an element of the SV40 late promoter in vectors used for expression studies in COS cells.
Author(s) -
Cattaneo R.,
Will H.,
Darai G.,
Pfaff E.,
Schaller H.
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01455.x
Subject(s) - biology , dna transposable elements , microbiology and biotechnology , promoter , vector (molecular biology) , gene expression , genetics , virology , gene , transposable element , recombinant dna , genome
Plasmids containing hepatitis B virus (HBV) DNA and a 232‐bp SV40 DNA fragment encoding the origin of replication were constructed. When introduced by transfection into COS cells, these plasmids directed the synthesis of hepatitis B surface antigen. S1 mapping of the mRNAs covering the S gene showed that transcriptional initiation was promoted by the interaction of HBV sequences with an SV40 promoter element: transcription started on HBV DNA but had several properties of SV40 late transcription. The detection of a promoter element in an SV40 origin fragment commonly used in the COS system is important for the interpretation of data deriving from expression studies in COS cells.