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Adenovirus cores can function as templates in in vitro DNA replication.
Author(s) -
Goding C.R.,
Russell W.C.
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01428.x
Subject(s) - biology , dna replication , dna , function (biology) , in vitro , virology , microbiology and biotechnology , genetics
Adenovirus cores prepared by gentle disruption of virus by heating at 56 degrees C in the presence of deoxycholate were able to function as templates in an in vitro DNA replication system, allowing both initiation, indicated by the formation of terminal protein‐dCMP complex, and elongation of > 300 nucleotides. Using both cores and DNA‐protein complexes as templates, it was also demonstrated that novobiocin, an inhibitor of DNA gyrase, inhibited in vitro DNA replication by preventing formation of the initiation complex.