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Construction of novel cytochrome b genes in yeast mitochondria by subtraction or addition of introns.
Author(s) -
Labouesse M.,
Slonimski P.P.
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01416.x
Subject(s) - intron , biology , gene , exon , genetics , group i catalytic intron , mitochondrial dna , nuclear gene , group ii intron , mt rnr1 , cytochrome b , microbiology and biotechnology , rna , rna splicing
The mitochondrial cob‐box gene coding for apocytochrome b in yeast has five introns and six exons or two introns and three exons depending on the wild‐type strain considered. Some intron mutations in this gene affect not only its expression but also that of another mitochondrial gene: oxi3. To understand better the function of introns in gene expression, we have constructed a series of new strains that differ only by the presence or absence of one of the five wild‐type introns in the cytochrome b gene, the rest of the mitochondrial and nuclear genome remaining unchanged. All constructions result from in vivo recombination events between rho‐ donor and rho+ recipient mtDNA. The following genes have been constructed: [see text]. Interestingly, all the genes lead to the synthesis of cytochrome b, while only the genes having the intron bI4 allow the expression of oxi3. A nuclear gene, when mutated, can compensate for the absence of the intron bI4.