Premium
Cloning of several cDNA segments coding for human liver proteins.
Author(s) -
Costanzo F.,
Castagnoli L.,
Dente L.,
Arcari P.,
Smith M.,
Costanzo P.,
Raugei G.,
Izzo P.,
Pietropaolo T.C.,
Bougueleret L.,
Cimino F.,
Salvatore F.,
Cortese R.
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01380.x
Subject(s) - biology , computational biology
A human cDNA library was constructed using M13 derivative vectors. The simple and rapid procedures for sequencing single‐stranded DNA by the dideoxy chain termination method allowed a screening of individual clones directly by DNA sequence analysis. Some of these clones were identified as coding for: serum albumin, alpha1‐antitrypsin, retinol‐binding protein, prothrombin, haptoglobin, and metallothionein. Furthermore, a clone coding for aldolase B was tentatively identified on the basis of high sequence homology with rabbit muscle aldolase.