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Organization of internucleosomal DNA in rat liver chromatin.
Author(s) -
Strauss F.,
Prunell A.
Publication year - 1983
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1983.tb01379.x
Subject(s) - biology , nucleosome , chromatin , dna , microbiology and biotechnology , nuclease , deoxyribonuclease i , exonuclease iii , hypersensitive site , apoptotic dna fragmentation , genetics , base sequence , gene , apoptosis , dna fragmentation , programmed cell death , escherichia coli
A detailed analysis of the length distribution of DNA in nucleosome dimers trimmed with exonuclease III and S1 nuclease suggests that the previously described variation of internucleosomal distance in rat liver occurs, at least for a subset of the nucleosomes, by integral multiples of the helical repeat of the DNA. Results obtained upon digestion of chromatin with DNase II further suggest that lengths of internucleosomal DNA are integral multiples of the helical repeat of the DNA plus approximately 5 bp. Restraints imposed by these features on the arrangement of nucleosomes along the fiber are discussed.