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A monoclonal antibody against a 135‐K Golgi membrane protein.
Author(s) -
Burke B.,
Griffiths G.,
Reggio H.,
Louvard D.,
Warren G.
Publication year - 1982
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1982.tb01364.x
Subject(s) - golgi apparatus , biology , immunoelectron microscopy , monoclonal antibody , vesicle , polyclonal antibodies , golgi membrane , copi , antiserum , microbiology and biotechnology , membrane protein , endoplasmic reticulum , membrane , antibody , biochemistry , secretory pathway , immunology
A monoclonal antibody (53FC3) has been produced against a Golgi membrane protein with a mol. wt. of 135 000 which was originally identified using a polyclonal antiserum. Treatment of isolated, intact Golgi vesicles with protease caused a decrease in mol. wt. of 5000‐10 000, whereas in the presence of Triton X‐100, the protein was completely degraded. This shows that the protein spans the bilayer and that most of its mass is on the luminal side of Golgi membranes. Using two immunoelectron microscopic techniques, the protein was found in one or two cisternae on one side of the Golgi stack which, in normal rat kidney cells, had 4‐6 cisternae. As an illustration of the use to which this monoclonal antibody can be put we present a light microscopic study of the disassembly and reassembly of the Golgi complex during mitosis.