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Recombinant retroviral DNA yielding high expression of hepatitis B surface antigen.
Author(s) -
Stratowa C.,
Doehmer J.,
Wang Y.,
Hofschneider P.H.
Publication year - 1982
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1982.tb01357.x
Subject(s) - hbsag , biology , recombinant dna , virology , microbiology and biotechnology , hepatitis b virus , genomic dna , dna , gene , virus , genetics
A genomic fragment of hepatitis B virus encoding the surface antigen (HBsAg) was inserted into the proviral genome of Moloney mouse sarcoma virus (MSV), obtained from the mouse cell line G8 ‐124. The recombinant DNA was introduced into NIH 3T3 mouse fibroblasts. Cells, morphologically transformed by the oncogene of MSV (v‐mosM) were selected, established as cell lines and tested for expression of HBsAg. An expression level of up to 4.5 micrograms/10(7) cells/day was detected.