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A site‐specific, conservative recombination system carried by bacteriophage P1. Mapping the recombinase gene cin and the cross‐over sites cix for the inversion of the C segment.
Author(s) -
Iida S.,
Meyer J.,
Kennedy K.E.,
Arber W.
Publication year - 1982
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1982.tb01336.x
Subject(s) - biology , recombinase , bacteriophage , recombination , genetics , site specific recombination , inversion (geology) , gene , chromosomal inversion , cre recombinase , microbiology and biotechnology , paleontology , transgene , chromosome , escherichia coli , structural basin , genetically modified mouse , karyotype
The bacteriophage P1 genome carries an invertible C segment consisting of 3‐kb unique sequences flanked by 0.6‐kb inverted repeats. With insertion and deletion mutants of P1 derivatives the site‐specific recombinase gene cin for C inversion) has been mapped adjacent to the C segment and the cix sites (for C inversion cross‐over) have been located at the outside ends of the inverted repeats. Inversion of the C segment functions as a biological switch and controls expression of the gene(s) responsible for phage infectivity carried on the C segment. The cin gene product can promote recombination between a ‘quasi‐ cix’ site on plasmid pBR322 and a cix site on P1 DNA. The junctions formed on the resulting co‐integrate can also serve as cix sites. This observation implies a potential evolutionary process to bring genes under the control of a biological switch acting by DNA inversion.