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Molecularly cloned c‐mos(rat) is biologically active.
Author(s) -
Hoorn F.A.,
Hulsebos E.,
Berns A.J.,
Bloemers H.P.
Publication year - 1982
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1982.tb01316.x
Subject(s) - biology , computational biology , genetics
A unique rat cellular gene, c‐mos(rat), homologous to the transforming sequences, v‐mos, of Moloney murine sarcoma virus (M‐MSV) was detected by hybridization to a v‐mos specific probe. The c‐mos(rat) gene was cloned together with its flanking sequences in an 11‐kbp EcoRI DNA fragment inserted in vector Charon 4A. Two probes were used to investigate the position and orientation of c‐mos(rat) in the clone examined (D3e), namely pMSV ‐31 which contains the sequences specific for the transforming sequences of M‐MSV and pCS‐1 which harbors 0.5 kbp of 5′‐terminal sequences of c‐mos(mouse) as well as 0.7 kbp of its flanking sequences. After ligation of a restriction fragment of clone D3e containing c‐mos(rat) to a fragment containing the long terminal repeat of M‐MSV and transfection of the DNA onto rat cells, we detected foci of transformed cells, thus showing that c‐mos(rat) is biologically active. Using DNA framents derived from clone D3e, we studied the conservation of c‐mos and of its flanking sequences in several species. c‐mos(rat) as well as some of its flanking sequences appeared to be highly conserved in the species studied.