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A cAMP‐dependent protein kinase is present in differentiating Dictyostelium discoideum cells
Author(s) -
Gunzburg J.de,
Veron M.
Publication year - 1982
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1982.tb01297.x
Subject(s) - biology , humanities , microbiology and biotechnology , philosophy
We demonstrate the occurrence of a cAMP‐dependent protein kinase in Dictyostelium discoideum cells at the terminal stage of differentiation. A cAMP‐binding component was purified to homogeneity by affinity chromatography. This subunit inhibits the activity of purified catalytic subunit from beef heart protein kinase; the inhibition is reversed upon addition of cAMP. The protein is highly specific for cAMP and has a dissociation constant of 4 nM. The isolated regulatory subunit is a monomer of 39 K, with a sedimentation coefficient of 3.5S and a frictional coefficient of 1.24. The differences between this regulatory subunit and regulatory subunits of protein kinases from other sources are discussed.