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Species‐specific monoclonal antibodies in the assignment of the gene for human fibronectin to chromosome 2.
Author(s) -
Zardi L.,
Cianfriglia M.,
Balza E.,
Carnemolla B.,
Siri A.,
Croce C.M.
Publication year - 1982
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1982.tb01273.x
Subject(s) - biology , monoclonal antibody , genetics , gene , chromosome , microbiology and biotechnology , antibody
Using three different species‐specific monoclonal antibodies we have studied, in human‐mouse and human‐hamster somatic cell hybrids, the correlation between the presence of different human chromosomes and the ability to release human fibronectin into the tissue culture medium. Presence of human fibronectin was determined by an affinity‐radioimmunoassay. In addition, tissue culture media of the different hybrids were separated on SDS‐polyacrylamide gels, the proteins were blotted onto a nitrocellulose sheet and human fibronectin visualized by an immunoenzymatic technique. Karyology and determination of isoenzyme markers of specific human chromosomes show that the ability to produce human fibronectin segregated with the presence of human chromosome 2.