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Differentiation of Ob17 preadipocytes to adipocytes: requirement of adipose conversion factor(s) for fat cell cluster formation.
Author(s) -
Grimaldi P.,
Djian P.,
Negrel R.,
Ailhaud G.
Publication year - 1982
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1982.tb01231.x
Subject(s) - adipose tissue , biology , fetal bovine serum , doubling time , cell , endocrinology , medicine , fraction (chemistry) , microbiology and biotechnology , biochemistry , chromatography , chemistry
An active fraction present in fetal calf serum (adipose conversion factor(s) or ACF) controls the formation of fat cell clusters observed during the adipose conversion of Ob17 cells. This conclusion is based upon microscopic examinations, autoradiographic experiments, and activity levels of characteristic enzyme markers. ACF has no effect on the doubling time of exponentially‐growing cells but is required in the first few days of the resting phase. ACF has been partially purified; it is a low mol. wt. component (less than 6000‐8000), proteas ‐insensitive, thermostable at neutral pH but not at strongly acidic pH. An active fraction recovered from bovine pituitary extract shows properties similar to those of ACF. Therefore, ACF plays the role of a mitogenic factor specific for cells susceptible to conversion to adipose cells.