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Immunoglobulin V region variants in hybridoma cells. I. Isolation of a variant with altered idiotypic and antigen binding specificity.
Author(s) -
Brüggemann M.,
Radbruch A.,
Rajewsky K.
Publication year - 1982
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1982.tb01219.x
Subject(s) - biology , antigen , antibody , microbiology and biotechnology , genetics
The hybridoma cell line B1‐8.delta 1 expresses an IgD antibody with specificity for the hapten 4‐hydroxy‐3‐nitro‐phenylacetyl (NP). Two monoclonal antibodies, each recognizing an idiotypic determinant (idiotope) in the variable region of antibody B1‐8, and fluorescence‐activated cell sorting were used for the isolation of spontaneous somatic cell variants whose antibody product lacks the expression of one of the two idiotopes. The variant cells carry a mutation which is expressed in the variable region of the antibody heavy chains and which reduces the affinity of the antibody for hapten greater than 100‐fold. Idiotope loss variants of the selected phenotype are present in the B1‐8.delta 1 cell population at low frequency.