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Regulated high efficiency expression of human interferon‐alpha in Saccharomyces cerevisiae.
Author(s) -
Tuite M.F.,
Dobson M.J.,
Roberts N.A.,
King R.M.,
Burke D.C.,
Kingsman S.M.,
Kingsman A.J.
Publication year - 1982
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1982.tb01215.x
Subject(s) - biology , saccharomyces cerevisiae , alpha interferon , alpha (finance) , genetics , interferon , microbiology and biotechnology , yeast , medicine , construct validity , nursing , patient satisfaction
The 5′ control region of the yeast phosphoglycerate kinase gene (PGK) was fused to the coding sequence of a human interferon‐alpha. This PGK‐interferon fusion was then introduced into yeast on a high copy number 2mu‐based plasmid vector. Strains containing this plasmid produced a PGK‐interferon‐alpha fusion protein as 1‐2% of cell protein and the expression of interferon activity was regulated by the availability of a fermentable carbon source. The system is capable of making as much as 15 mg of human interferon‐alpha per litre of batch culture.