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Nucleosome reconstitution on plasmid‐inserted poly(dA) . poly(dT).
Author(s) -
Prunell A.
Publication year - 1982
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1982.tb01143.x
Subject(s) - insert (composites) , nucleosome , biology , plasmid , chromatin , recombinant dna , histone , particle (ecology) , microbiology and biotechnology , dna , genetics , biophysics , materials science , gene , ecology , composite material
Chromatin was reconstituted from core histones and recombinant plasmid DNAs carrying poly(dA) . poly(dT) inserts of various lengths. A 97‐bp insert was found to occupy discrete and regularly‐spaced positions on the edges of the nucleosome. This insert cannot, however, be entirely included due to a block in the center of the particle. In contrast, nucleosomes reconstitute on a shorter 20‐bp insert. In this case, the insert shows a marked preference for the edges of the particle. Possible structural and physiological implications of these observations are discussed.