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The genes coding for the cytoskeletal proteins actin and vimentin in warm‐blooded vertebrates.
Author(s) -
Dodemont H.J.,
Soriano P.,
Quax W.J.,
Ramaekers F.,
Lenstra J.A.,
Groenen M.A.,
Bernardi G.,
Bloemendal H.
Publication year - 1982
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1982.tb01142.x
Subject(s) - biology , cytoskeleton , vimentin , gene , actin , genetics , microbiology and biotechnology , intermediate filament , computational biology , cell , immunohistochemistry , immunology
Recombinant plasmids were made containing cDNAs synthesized on hamster mRNAs coding for cytoskeletal (beta‐ or gamma‐) actins and for vimentin. Hybridization of the actin probe on restriction digests of one avian and five mammalian DNAs yielded multiple bands; the vimentin probe revealed only one band (accompanied by 2‐3 faint bands in some DNAs). The results obtained with the vimentin probe indicate that the corresponding coding sequences: (a) are highly conserved in warm‐blooded vertebrates like the actin sequences; (b) have strongly diverged from those coding for other intermediate filament proteins, since hybridization of the vimentin probe does not lead to a diagnostic multiband pattern; and (c) most likely contribute to single gene, in contrast to the sequences coding for other cytoskeletal proteins. Hybridization of the probes on mRNAs from the different sources used showed that the non‐coding sequences of both vimentin and actin genes are conserved in length.